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PR0100
Sigma
Primer Library, Arabidopsis Inducible Genes, Set
Description
| Components | • Primer Library for Q-RT-PCR of Arabidopsis Genes, Product Code P5621. A 96-well plate containing 96 pairs of gene specific primers as lyophilized powder. • Corning® Microcentrifuge Tubes, Product Code Z372226. A rack of 96 individual tubes in 96-well format • Caps for Corning Microcentrifuge Tubes, Product Code C5481. 96 caps in strips of 8, for rack of 96 tubes |
| Application | The primer set (2 nmole for each primer) in each well is lyophilized. Before use, dissolve the primers in 200 mL TE buffer (10 mM Tris, pH 8.0, 0.001 mM EDTA) and transfer into individual tubes provided. The tubes should be prearranged in 96-well format on the provided rack in the same order as that of the plate. Cap each tube carefully. |
| General description | The Primer Library for Arabidopsis Pathogen-inducible Genes is designed for expression analysis of Arabidopsis defense response genes by quantitative RT-PCR (Q-RT-PCR). This primer library contains 96 sets of gene-specific primers that can be used for expression profiling of 96 genes, of which 93 are pathogen-inducible genes and 3 are housekeeping genes. The pathogen-inducible genes are selected based on a search of an Arabidopsis database and microarray analysis. The housekeeping genes, Actin-2, CBP20, and Ubiquitin, are selected based on their expression patterns. The Actin-2 gene is designed for high abundance genes, whereas CBP20 and Ubiquitin genes are designed for medium to low abundance genes. All of the control genes are constitutively expressed in different tissues. All primers in the library are suitable for one-step or two-step RT-PCR and for both real time and non-real time RT-PCR analysis. In addition, the specificity and amplification efficiency of all primers have been validated using pathogen-infected Arabidopsis leaf samples. The primer library, combined with Sigma's robust RT-PCR reagents, provides cost effective, accurate, and non-chip based assays for expression profiling of a set of pathogen-inducible genes in Arabidopsis. Some of the genes included in the library can also be used as signature genes to characterize disease-resistance mutants. Primers are packaged in a 96-well plate format. Each well contains a lyophilized set of forward and reverse primers for amplification of a specific gene. Each primer set has enough quantity for 200 RT-PCR reactions (20-25 μl reaction volume). |
Properties
| storage temp. | −20°C |
